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首页> 外文OA文献 >Screening Antitumor Compounds Psoralen and Isopsoralen from Psoralea corylifolia L. Seeds
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Screening Antitumor Compounds Psoralen and Isopsoralen from Psoralea corylifolia L. Seeds

机译：从补骨脂种子中筛选抗肿瘤化合物补骨脂素和异补骨脂素

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Psoralea corylifolia L. (Fabaceae) is a widely used medical plant in China. This study was designed to screen and identify bioactive compounds with anticancer activity from the seeds of Psoralea corylifolia L. One volatile fraction (fraction I) and three other fractions (fraction II, III, IV) from methanol extraction of P. corylifolia L. were obtained. Bioactivities of these fractions were evaluated by the cytotoxicity on KB, KBv200, K562, K562/ADM cancer cells with MTT assay. Major components in the active fraction were identified by HPLC/MSn. Fraction IV significantly inhibits the growth of cancer cells in a dose-dependent manner. The IC50 values were 21.6, 24.4, 10.0 and 26.9, respectively. Psoralen and isopsoralen, isolated from fraction IV, were subject to bioactive assay and presented a dose-dependent anticancer activity in four cancer cell lines (KB, KBv200, K562 and K562/ADM). The IC50 values of psoralen were 88.1, 86.6, 24.4 and 62.6, which of isopsoralen were 61.9, 49.4, 49.6 and 72.0, respectively. Apoptosis of tumor cell significantly increased after treated with psoralen and isopsoralen. Induction of apoptotic activity was confirmed by flow cytometry after staining with Annexin V/PI. These results suggested psoralen and isopsoralen contribute to anticancer effect of P. corylifolia L.
机译：Psoralea corylifolia L.（Fabaceae）是在中国广泛使用的药用植物。这项研究旨在从苦瓜种子中筛选和鉴定具有抗癌活性的生物活性化合物。分别从甲醇提取的苦瓜中提取出一个挥发性组分（组分I）和其他三个组分（组分II，III，IV）。获得。通过MTT测定，通过对KB，KBv200，K562，K562 / ADM癌细胞的细胞毒性评估这些级分的生物活性。活性级分中的主要成分通过HPLC / MSn鉴定。级分IV以剂量依赖性方式显着抑制癌细胞的生长。 IC50值分别为21.6、24.4、10.0和26.9。分离自级分IV的补骨脂素和异补骨脂素经过生物活性测定，在四种癌细胞系（KB，KBv200，K562和K562 / ADM）中表现出剂量依赖性的抗癌活性。补骨脂素的IC50值分别为88.1、86.6、24.4和62.6，而异补骨脂素的IC50值分别为61.9、49.4、49.6和72.0。补骨脂素和异补骨脂素处理后，肿瘤细胞的凋亡明显增加。在用膜联蛋白V / PI染色后，通过流式细胞术证实了凋亡活性的诱导。这些结果表明补骨脂素和异补骨脂素有助于紫花苜蓿的抗癌作用。

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